Cytomimetic Large-Scale Vesicle Aggregation and Fusion Based on Host-Guest Interaction
作者：Jin, H. B.; Liu, Y.; Zheng, Y. L.; Huang, W.; Zhou, Y.F.*; Yan, D.Y.
關(guān)鍵字：Vesicle, Aggregation, Fusion,Host-Guest Interaction
論文來源：期刊
具體來源：Langmuir 2012, 28, 2066. http://pubs.acs.org/doi/pdf/10.1021/la203857s
發(fā)表時間：2011年
Herein, we have shown a
large-scale cell-mimetic (cytomimetic) aggregation process by using cell-sized
polymer vesicles as the building blocks and intervesicular host–guest molecular
recognition interactions as the driving force. We first prepared the
hyperbranched polymer vesicles named branched polymersomes (BPs) around 5–10 μm
through the aqueous self-assembly of a hyperbranched multiarm copolymer of
HBPO-star-PEO [HBPO = hyperbranched poly(3-ethyl-3-oxetanemethanol); PEO =
poly(ethylene oxide)]. Subsequently, adamantane-functionalized BPs (Ada-BPs) or
β-cyclodextrin-functionalized BPs (CD-BPs) were prepared through the coassembly
of HBPO-star-PEO and Ada-modified HBPO-star-PEO (HBPO-star-PEO-Ada), or of
HBPO-star-PEO and CD-modified HBPO-star-PEO (HBPO-star-PEO-CD), respectively.
Macroscopic vesicle aggregates were obtained by mixing CD-BPs and Ada-BPs. The
intervesicular host–guest recognition interactions between β-CD units in CD-BPs
and Ada units in Ada-BPs, which were proved by 1H nuclear Overhauser
effect spectroscopy (NOESY) spectrum and the fluorescence probe method, are
responsible for the vesicle aggregation. Additionally, the vesicle fusion events
happened frequently in the process of vesicle aggregation, which were certified
by double-labeling fluorescent assay, real-time observation, content mixing
assay, and component mixing assay.