報(bào)告主題：DNA與聚陽離子復(fù)合物: 從基因傳遞載體到原始細(xì)胞模型
報(bào)告人：梁德海 教授
主持人：陳永明 教授
時(shí)間：2015年12月29日（星期二）上午09:30-10:30
地點(diǎn)：豐盛堂A403
報(bào)告摘要：
研究DNA/RNA 與聚陽離子的靜態(tài)和動(dòng)態(tài)復(fù)合行為對(duì)于探討聚電解質(zhì)的相互作用機(jī)理，開發(fā)高效基因傳遞載體，以及研究原始細(xì)胞的動(dòng)態(tài)模型都有重要意義。在靜態(tài)復(fù)合中，我們重點(diǎn)探討了DNA復(fù)合物的長程動(dòng)力學(xué)行為，發(fā)現(xiàn)歧化是影響復(fù)合物動(dòng)力學(xué)的主要因素。通過調(diào)控歧化速率，構(gòu)筑了動(dòng)力學(xué)控制的膠囊結(jié)構(gòu)。在動(dòng)態(tài)復(fù)合中，首先模擬血液流動(dòng)引起的剪切作用，探討了外加剪切力對(duì)DNA復(fù)合物穩(wěn)定性的影響，并據(jù)此開發(fā)了具有肺靶向功能的siRNA傳遞載體。 接下來，利用電場來調(diào)控DNA與聚陽離子在非平衡態(tài)的復(fù)合行為。結(jié)果表明，二者形成的復(fù)合物發(fā)生了周期性的內(nèi)分區(qū)化，尺寸和形狀的動(dòng)態(tài)漲落，混沌式的增長和融合等許多與生命活動(dòng)類似的行為。在周期性的內(nèi)分區(qū)化過程中還伴隨著物質(zhì)的運(yùn)輸，主要表現(xiàn)為聚陽離子含量的減少。我們用電場力學(xué)引起的聚電解質(zhì)復(fù)合物的解散，繼而引起的滲透壓變化來解釋內(nèi)分區(qū)化行為。這些結(jié)果都表明在非平衡態(tài)下研究復(fù)合物粒子的動(dòng)態(tài)行為對(duì)于開發(fā)原始細(xì)胞，探索生命起源具有指導(dǎo)意義。
報(bào)告人簡介：
EDucation
2001 Ph.D. Department of Chemistry, State University of New York at Stony Brook, NY, USA (Thesis Advisor: Benjamin Chu)
1996 M.S. (Candidate), Department of Chemistry, Nankai Unversity, Tianjin, China
1994 B.S. Department of Environmental Sciences, Nankai Unversity, Tianjin, China
Employment
2012-present: Professor, College of Chemistry, Peking University, Beijing, China
2006-2012: Associate Professor, College of Chemistry, Peking University, Beijing, China
2001-2005: Postdoctoral Research Associate, Department of Chemistry, State University of New York at Stony Brook, NY, USA
Research Interests
1. Electric field excitation and non-equilibrium dynamics in Peptide/DNA protocells
2. Anisotropic assembly regulated by macromolecular crowding and confinement
3. Peptide mediated endocytosis of biomembrane
4. Enzyme reaction in polyelectrolyte complex
5. Lung-targeting siRNA delivery vehicles
Teaching Reponsibility
Polymer physics: structures and properties, 2007-present
Today’s Chemistry, 2010-present
Comprehensive Experiments: DNA complex, 2009-2012
Literature Seminar, 2007-2015
Honors &Awards
2015 The 9th Feng Xinde Polymer Prize, Elsevier
2010 Winner of Education Ministry''''''''s New Century Excellent Talents Supporting Plan
2001 President’s Award to Distinguished Doctoral Students, SUNY at Stony Brook
2001 Lee Myer’s Outstanding Graduate Student Award, SUNY at Stony Brook
2000 Excellence in Research Award, Sigma Xi, the Scientific Research Society
Publicatons
110 Papers, 2 US patents and 4 Chinese patents.