Development of technologies for biomedical detectionplatform is critical to meet the global challenges of various diseasediagnoses, especially for point-of-use applications. Because of its naturalsimplicity, effectiveness, and easy repeatability, random covalent-bindingtechnique is widely adopted in antibody immobilization. However, itsantigen-binding capacity is relatively low when compared to site-specificimmobilization of antibody. Herein, we report that a detection platformmodified with boronic acid (BA)-containing sulfobetaine-based polymerbrush. Mainly because of the advantage of oriented immobilization ofantibody endowed with BA-containing three-dimensional polymer brusharchitecture, the platform had a high antigen-binding capacity. Notably,nonspecific protein adsorption was also suppressed by the zwitterionicpendants, thus greatly enhanced signal-to-noise (S/N) values for antigenrecognition. Furthermore, antibodies captured by BA pendants could be released in dissociation media. This new platform ispromising for potential applications in immunoassays.
