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 | 濉戞枡 | 姍¤兌 | 綰ょ淮 | 娑傛枡涓庢補(bǔ)澧?/a> | 鑳剁矘鍓?/a> | 澶嶅悎鏉愭枡 | 鐭沖寲 | 鍖栧伐 | 浠櫒浠〃 | 鏂頒駭涓?/a> | 涓撻 | 榪斿洖棣栭〉 |
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| 濉戞枡 | 姍¤兌 | 綰ょ淮 | 娑傛枡涓庢補(bǔ)澧?/a> | 鑳剁矘鍓?/a> | 澶嶅悎鏉愭枡 | 鐭沖寲 | 鍖栧伐 | 浠櫒浠〃 | 鏂頒駭涓?/a> | 涓撻 | 榪斿洖棣栭〉 |
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鐗瑰畾鍩哄洜紿佸彉鍙兘浼?xì)瀵艰嚧鑲跨槫缁嗚優(yōu)漶旂敓鐗箤D婅涓猴紝渚嬪錛岃秴寮鴻愯嵂鎬х瓑銆傚湪媧葷粏鑳?yōu)鍐呫佸崟緇嗚優(yōu)姘村鉤涓婃帰绱㈠熀鍥犵獊鍙樹(shù)笌媧葷粏鑳?yōu)琛屼负鐩稿呄x(chóng)ц兘澶熶負(fù)涓村簥娌葷枟鎻愪緵鏇村姞綺懼噯鍙潬鐨勫弬鑰冦?
榪戞棩錛屽寳浜埅絀鴻埅澶╁ぇ瀛﹀父鍑屼咕璇鵑緇勫湪钁楀悕鏈熷垔Nano Letters涓婂彂琛ㄤ簡(jiǎn)涓綃囧悕涓衡淪ingle Living Cell Analysis Nano-platform for High-throughput Interrogation of Gene Mutation and Cellular Behavior鈥?鐨勭爺絀惰鏂囥傚茍琚変負(fù)褰撴湡鐨勫唴灝侀潰鏂囩珷銆傚紑鍙戜簡(jiǎn)涓縐嶆柊鍨嬫椿緇嗚優(yōu)闃靛垪鐮旂┒騫沖彴錛岄氳繃綰崇背鐢?shù)閫掗佹妧鏈皢涓縐嶅彲鍘熶綅淇″彿鏀懼ぇ鐨凞omino鑽у厜鎺㈤拡楂樻晥鍦伴掗佽嚦媧葷粏鑳?yōu)鍐呭Q屽湪鍗曠粏鑳?yōu)姘打q蟲(chóng)嫻嬬粏鑳?yōu)鍐呭熀鍥牻H佸彉銆傚悓鏃惰姱鐗囧叿澶囩粏鑳?yōu)瀵诲潃鐨勫姛鑳斤紝鍙疄鐜拌偪鐦ょ粏鑳?yōu)鑰愯嵂鎬х殑鍘熶綅鍒嗘瀽錛堝浘1錛夈傚湪鐮旂┒涓村簥鑲虹檶緇嗚優(yōu)鏍鋒湰鏃跺彂鐜幫紝鑲虹檶鏌愪簺鍩哄洜紿佸彉緇嗚優(yōu)姣斾緥涓庣粏鑳?yōu)瀵归澏鍚戣嵂鏁忔劅绋嬪害鍛堢幇姝ｇ浉鍏虫с傛騫沖彴涓哄崟緇嗚優(yōu)鍒嗘瀽銆佽優(yōu)鍐呭熀鍥犳嫻嬫彁渚涗簡(jiǎn)涓縐嶉氱敤鏂規(guī)硶錛屽湪鎸囧涓村簥綺懼噯娌葷枟鏂歸潰鍏鋒湁杈冨ぇ鐨勫簲鐢ㄦ綔鍔涖?
鍥?. 鍗曠粏鑳?yōu)鍒嗘瀽绾尘c沖鉤鍙板師鐞嗙ず鎰忓浘銆傦紙a錛夊熀浜庡井瀛旈樀鍒楃殑綰崇背鑺墖鍦ㄥ崟涓椿緇嗚優(yōu)涓嫻嬮澏鏍嘡NA鐨勫師鐞嗐傦紙b錛塂omino鎺㈤拡涓庨澏鏍嘡NA緇撳悎鍓嶏紙OFF錛夊悗錛圤N錛夌殑鏋勮薄鍙樺寲紺烘剰鍥俱傦紙c錛夌撼綾寵姱鐗囩敤浜庡皢Domino鎺㈤拡閫掗佸埌媧葷粏鑳?yōu)涓Q屽茍鎻愪緵姣忎釜緇嗚優(yōu)鐨勪綅緗俊鎭傦紙d錛夎姱鐗囧垎灞傝閰嶇ず鎰忓浘銆?/span>
EGFR紿佸彉鍦ㄨ偤鐧屽熀鍥犵獊鍙樹(shù)腑瀛樺湪鐜囨渶楂樸傚敖綆GFR闈跺悜鑽墿鍙湁鏁堝歡闀挎?zhèn)ｈ呭瓨?gòu)z繪湡錛屼絾鑲跨槫緇嗚優(yōu)鏄撲駭鐢熻愯嵂鎬э紝閫犳垚闈跺悜鑽墿澶辨晥銆傞嫻嬫?zhèn)ｈ呮槸鍚﹀瓨鍦‥GFR紿佸彉錛屽茍鎺ㄦ柇鍏惰偪鐦ょ粏鑳?yōu)瀵归澏鍚戣嵂鐗╃殑鑰愯嵂鎬э紝鍦ㄦ彁楂樿偤鐧屾不鐤楁晥鏋滀腑璧風(fēng)潃鍏抽敭浣滅敤銆備綔鑰呰璁′簡(jiǎn)涓縐嶅悕涓衡淒omino鈥濈殑DNA鎺㈤拡銆傛鎺㈤拡鍒╃敤紕卞熀浜掕ˉ閰嶅鍘熷垯錛岄氳繃灝嗘垚瀵圭殑鍙戝すDNA搴忓垪緇勮褰㈡垚绱у瘑鐨勮灪鏃嬮摼銆備笌浼犵粺鐨勫垎瀛愪俊鏍囩浉姣旓紝Domino鎺㈤拡琚澏鏍嘡NA璇嗗埆鐨勫弽搴旈熷害鍔犲揩浜?鍊嶏紝鑽у厜淇″彿澧炲己浜?0鍊嶃備負(fù)閫掗佹鎺㈤拡錛屼綔鑰呭紑鍙戜簡(jiǎn)涓縐嶇撼綾沖瓟鐢?shù)閫掗佽姱鐗囷紝灝咲omino鎺㈤拡閫掗佸埌浣嶄簬鍙鍧寰瓟涓殑鏁扮櫨涓囦釜鐨勮偤鐧岀粏鑳?yōu)涓Q屽疄鐜扮粏鑳?yōu)鍐呯殑EGFR鍩哄洜紿佸彉?gòu)個(gè)嫻嬶紙鍥?錛夈?
鍥?. 綰崇背騫沖彴媯(gè)嫻嬬粏鑳?yōu)鍐呯殑鍩哄洜绐佸彉銆傦紙a錛夊井瀛旈樀鍒楅澏鍚慙858R紿佸彉鐨凞omino鎺㈤拡媯(gè)嫻婬1975緇嗚優(yōu)錛圗GFR L858R紿佸彉錛夈傛瘮渚嬪昂錛?0 渭m銆傦紙b錛夌撼綾沖鉤鍙版嫻嬭偤鐧岀粏鑳?yōu)H1975錛圠858R紿佸彉錛夛紝HCC827錛?9澶栨樉瀛愮己澶辯獊鍙橈級(jí)鍜孉549錛堥噹鐢熷瀷錛塃GFR L858R鍜?9澶栨樉瀛愮己澶辯獊鍙樼殑鑽у厜鍥俱傛瘮渚嬪昂錛?0 渭m銆傦紙c錛夛紝錛坉錛夊拰錛坋錛夌撼綾沖鉤鍙版嫻嬭偤鐧岀粏鑳?yōu)H1975錛坈錛夛紝HCC827錛坉錛夊拰A549錛坋錛塃GFR L858R鍜?9澶栨樉瀛愮己澶辯獊鍙樼殑嫻佸紡緇撴灉銆傦紙f錛夐噰鐢ㄦ祦寮忕粏鑳?yōu)淙A媯(gè)嫻嬬殑H1975錛孒CC827鍜孉549緇嗚優(yōu)鐨勭獊鍙樼粏鑳?yōu)闃虫х巼銆傦紙g錛夌粺璁″井瀛旈樀鍒椾笂紿佸彉緇嗚優(yōu)鐨勯槼鎬х巼銆傦紙h錛塖LCA綰崇背騫沖彴閽堝H1975錛孒CC827鍜孉549緇嗚優(yōu)鐨勫姞杞芥晥鐜囷紝閫掗佹晥鐜囧拰緇嗚優(yōu)媧葷巼銆?/span>
浣滆呭埄鐢ㄨ騫沖彴鐮旂┒浜?jiǎn)鑲虹檶鎮(zhèn)ｈ呬復(fù)搴婃牱鍝佷腑鍗曚釜媧葷粏鑳?yōu)鐨勫熀鍥牻H佸彉錛屾彮紺轟簡(jiǎn)EGFR紿佸彉錛?1澶栨樉瀛怢858R鍜?9澶栨樉瀛怑746-A750緙哄け紿佸彉錛変笌鐩稿簲鐨勯澏鍚戣嵂鐗╄愬彈鎬т箣闂村瓨鍦ㄦ槑鏄劇殑姝ｇ浉鍏蟲(chóng)э紙鍥?3錛夈傝宸ヤ綔涓彁鍑虹殑綰崇背騫沖彴瀹炵幇鍘熶綅緇嗚優(yōu)鍩瑰吇錛屾椿緇嗚優(yōu)鍐呭熀鍥犳帰嫻嬩互鍙?qiáng)鏃督I哄彲鎺х殑緇嗚優(yōu)琛屼負(fù)鍒嗘瀽銆?
鍥?. 鍘熶唬鑲跨槫緇嗚優(yōu)鏍峰搧涓璄GFR RNA紿佸彉?gòu)個(gè)嫻嬪拰鑰愯嵂鎬у垎鏋愩傦紙a錛夌撼綾沖鉤鍙版嫻嬫?zhèn)ｈ匧1鍘熶唬鑲跨槫緇嗚優(yōu)涓璄GFR L858R紿佸彉RNA鐨勮崸鍏夊浘鍍忋傛瘮渚嬪昂錛?00 渭m銆傦紙b錛夊拰錛坈錛夐噰鐢ㄧ撼綾沖鉤鍙頒粠鍏鋒湁EGFR L858R紿佸彉錛坆錛夋垨19澶栨樉瀛愮己澶辯獊鍙橈紙c錛夌殑鍘熶唬鑲跨槫緇嗚優(yōu)鏍峰搧涓嫻嬪埌鐨勭獊鍙樼粏鑳?yōu)闃虫х巼銆傦紙d錛夊拰錛坋錛夊垎鍒EGFR L858R紿佸彉錛坉錛夋垨19澶栨樉瀛愮己澶辯獊鍙橈紙e錛夌殑鍘熶唬鑲跨槫緇嗚優(yōu)鏍峰搧榪涜鍗曠粏鑳?yōu)鍩哄洜鍒嗘瀽鐨剄PCR緇撴灉銆傦紙f錛夊拰錛坓錛塃GFR紿佸彉闈跺悜鑽墿鍘勬礇鏇垮凹鍜屽悏闈炴浛灝煎EGFR L858R紿佸彉錛坒錛夋垨19澶栨樉瀛愮己澶辯獊鍙橈紙g錛夌殑鍘熶唬鑲跨槫緇嗚優(yōu)鐨勬姂鍒朵綔鐢ㄣ?/span>
璇ユ枃绔犵涓/閫氳鍗曚綅涓哄寳鑸敓鐗╁尰瀛﹀伐紼嬮珮綺懼皷鍒涙柊涓績(jī)鍜岀敓鐗╁尰瀛﹀伐紼嬪闄€傜涓浣滆呮槸钁ｅ啀鍐嶅崥澹傚寳浜ぇ瀛﹂檮灞炶偪鐦ゅ尰闄㈠惔妤犳暀鎺堝拰棣栭兘鍖葷澶у闄勫睘鍖椾含涓栫邯鍧涘尰闄㈣枦鏂伴鏁欐巿涓哄叡鍚岄氳浣滆呫?
Reference: Z. Dong et al. Single Living Cell Analysis Nanoplatform for High-Throughput Interrogation of Gene Mutation and Cellular Behavior, Nano Letters, 2021, DOI: 10.1021/acs.nanolett.1c00199
钁ｅ啀鍐嶏細(xì)Nano Letters錛堝寳鑸狢HANG) .PDF